SAMPLE HANDLING PROCEDURE

 

 

 

 

 

 

 

 

 

 

Copy No.: Master Copy

Holder: Quality Manager

Issue No.: 01

Issue Date:

 

AMENDMENT SHEET

Sr. No.

Page No.

Section/Clause No.

Date of Amendment

Amendments Made

Reasons

Signature

Of QM

Signature of LD

               

               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               
               

SAMPLE HANDLING MANUAL

TABLE OF CONTENT

SL NO

TITLE

REVISION NO

LAST REVISION MADE

PAGE NO

 

COVER PAGE

00

00

01

 

AMMENDMENT SHEET

00

00

02

A

 

00

00

 

1

LIST OF LABORATORY EXAMINATIONS

00

00

05

2

COPY OF CONSENT FORM

00

00

 

3

PATIENT PREPARATION

00

00

06-09

B

PROCEDURES

 

ANTICOAGULANTS & CONCENTRATION

00

00

10

1

PRIMARY SAMPLE REQUIREMENT AND STORAGE

00

00

11-12

2

SPECIAL TIMING OF COLLECTION & LABELLING

00

00

13 - 14

3

SAFE DISPOSAL & RETENTION SCHEDULE

00

00

15-18

C

INSTRUCTIONS-I

1

PREVENTION OF NSI & ACIDENTS

00

00

29 - 24

TABLE OF CONTENT

SL NO

TITLE

REVISION NO

LAST REVISION MADE

TOTAL NO PAGES

2

  • ADA CRITERIA INCLUDING GTT GUIDELINES 2014

00

00

ANNEX 1 3

LIST OF LABOATORY EXAMINATION

  1. HEMATOLOGY
  1. Hemoglobin
  2. Total WBC Count
  3. Differential WBC count
  4. RBC count
  5. Platelet count
  6. Reticulocyte count
  7. Malaria Parasite (Smear)
  8. Malaria antigen
  9. Erythrocyte Sedimentation Rate(ESR)
  10. Haematocrit (PCV)
  11. Coomb’s Test (Direct)
  12. Coomb’s Test (Indirect)
  13. Absolute Values (MCH,)
  14. Absolute Values (MCHC)
  15. Absolute Values (MCV)
  16. Peripheral Smear Examination
  17. Absolute Eosinophil Count
  18. Blood Group and Rh.

 

 

 

 

 

 

 

 

 

 

 

 

  1. CLINICAL PATHOLOGY
  1. Urine- Routine Examination
  2. Stool- Routine Examination
  3. Stool Examination for Occult Blood.
  4. Semen analysis.
  5. 24 hrs urinary protein.
  6. Bence-Jones protein.

  1. BIO-CHEMISTRY
  1. Sugar
  2. Urea
  3. Creatinine
  4. Total & conjugate Bilirubin
  5. Total Protein
  6. Albumin
  7. SGOT
  8. SGPT
  9. Alkaline Phosphatase
  10. Cholesterol
  11. Triglyceride
  12. HDL Cholesterol
  13. T3
  14. T4
  15. TSH
  16. Sodium
  17. Potassium
  18. Uric Acid #9; #9;
  19. GGT
  20. Lipase
  21. Amylase.
  22. Phosphorus.
  23. Calcium (Total)
  24. Spot Urinary Micro albumin

Patient preparation

I. PATIENT PREPARATION FOR BLOOD SAMPLES:-

a) Fasting glucose:

Preparation: This test requires an overnight fast. One should wait to eat and/or take a hypoglycemic

agent (insulin or oral medication) until after sample has been drawn, unless told otherwise.

Overnight fasting - minimum 8 hrs is required ( Maximum 10 hrs.)

Delayed Fasting – As asked by the Referring Clinician / Doctor.

b) Lipid Profile

Fasting period preferably 12 hrs ; minimum 9 hours.

c) Post prandial glucose:

Blood specimen is collected 2 hrs after meal is taken.

d) Glucose Tolerance test:

* see Annex 1 the end of this manual

e) Timed collections:

For some hormonal studies, specimen is collected at specific timings. For S. cortisol : Blood specimen is collected at 8.00 AM & 4.00 PM. The timing of blood collection has clinical significance and hence must be clearly specified on the container.

General instruction for patients scheduled for phlebotomy ;

Following things may affect test results, which should be kept in mind.

 

 

 

 

 

 

 

 

 

 

 

II. PATIENT PREPARATION FOR URINE SAMPLES :-

a) Random Urine specimen:-

b) Timed urine specimen

c) 24 hrs urine collection

d) Urine collection instruction about pediatric patient

III. STOOL FOR OCCULT BLOOD

Patient is instructed to avoid the following food items and drugs for 3 (three) days before and during the stool collection period :-

Collection of Stool

IV. BODY FLUIDS

V. SEMEN

Just before you collect:

Note: Semen collected in a condom or by coitus interrupts is NOT ACCEPTABLE for Evaluation.

VI) Sputum:

Specimen: Early morning specimen in a sterile disposable wide mouthed plastic container is preferred which should be collected in a container provided by Kale Pathology Laboratory. Random sample can also be accepted.

Instruction to the patient:

Instructions for phlebotomist:

Blood collection technique (venous puncture)

Non-evacuated container:

Prolongated tourniquet application may cause haemoconcentration and can also increase serum enzymes , proteins and protein bound substances including cholesterol , calcium , TG.

Measures to avoid haemolysis

Haemolysis is a serious source of unreliable results. Haemolysis can be avoided by the following:

Identification of Primary Sample:

Type of specimen

Samples are identified as below:-

All samples are identified by an unique ID No. and first letter of patient’s name and surname.

Container & anticoagulants used for blood collection:

Container

Anticoagulant

Tests

Red/Brown top - Sticker

-

Biochemistry, serology,etc.

Grey top / Sticker

NaF

Plasma glucose.

Violet top- Sticker

EDTA

Haematological inestigations

Blue top- Sticker

Sodium citrate( 3.2 %)

Coagulations test

Black top- Sticker

Sodium citrate ( 3.8 %)

ESR

Anticoagulants and their concentration

  1. E.D.T.A. (Di-Potassium Salt): 1.5 +/- 0.25 mg/ml.

Method of preparation of vials in laboratory:

Di Potassium E.D.T.A. Salt #9; 3 g

Distilled Water #9; #9; 100 ml

Dispense 100µl in each vial for 2ml of blood. Allow the water content to evaporate at room temperature by placing the vials without cap in a tray. Cover the tray with a thin cloth.

Effect of excess of EDTA:

  1. Sodium Fluoride

Method of preparation of vials in laboratory:

Sodium Fluoride 2 gm

Potassium Oxalate 6 gm

Distilled Water 100 ml

Dispense 100µl in each vial for 2ml of blood. Allow the water content to evaporate at room temperature by placing the vials without cap in a tray. Cover the tray with a thin cloth.

3. Heparin: 10-20 I.U. / ml blood

Heparin is available as 5000 I.U. per 5 ml.

Dispense 30 µl in each vial for 2ml of blood. Allow the water content to evaporate at room temperature by placing the vials without cap in a tray. Cover the tray with a thin cloth.

4. Tri-sodium Citrate: 3.2 g %

Method of preparation in laboratory:

Na3C6H5O7, 2H2O 16 g

Distilled water #9; 500ml

(3.2g / 100ml)

URINARY DETERMINATION & PRESERVATIVE USED:

Determination

Preservative

Albumin

Boric acid (10-15 gm).

Bence Jones protein

Boric acid (10-15 gm).

Creatinine

Boric acid (10-15 gm).

Protein (24 hrs collection)

Boric acid (10-15 gm).

 

Sputum:

Collection of effusions or fluids like pleural, pericardial, ascitic, hydrocele fluids as a rule done by the clinical consultants.

PRIMARY SAMPLE STORAGE REQUIREMENT TEST WISE:

SL.

NO.

TEST

SPECIMEN/

AMOUNT

ADDITIVES

CONTAINER

STORAGE /STABILITY

1

Alanine aminotransferase ALT / SGPT.

2 ml clotted blood.

-

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature for 12-24 hrs.

Stable in serum at

2-8°c up to 3weeks

2

Albumin

2 ml clotted blood.

Plain Vial

With white

Sticker..

Stable in whole blood at room temp or at 2-8°c up to 8 hrs.

Stable in serum at

2-8°c for up to 2 weeks.

3

Alkaline phosphatase

2 ml clotted blood.

-

Plain Vial

With white

Sticker.

Stable in whole blood at room temp up to 12hrs.

Stable in serum at 4°C up to 1weeks

4

Aspartate aminotransferase AST / SGOT

2 ml clotted blood

-

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature for 12-24 hrs.

Stable in serum at

2-8°c up to 3weeks

5

Total amylase

/

Lipase

2 ml clotted blood.

Plain Vial

With white

Sticker.

Good stability in whole blood. Stable in serum at room temperature up to 1 weeks.

6

Bilirubin,total

/

Bilirubin,Direct

2 ml clotted blood.

Plain Vial

With white

Sticker.

Protect from light. Stable in whole blood at room temperature for 3 hrs.

Stable in serum at 2-8°C up to 1weeks.

7

Total Calcium

2 ml clotted blood. Collect preferably fasting blood and avoid venous stasis.

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature or at 2-8°C up to 3hrs.

Stable in serum at

2-8°C up to 72 hrs

8

Cholesterol /LDL Cholesterol /

HDL Cholesterol.

2 ml clotted blood.

Plain Vial

With white

Sticker.

Stable in whole blood at room temp or at 2-8°C up to 12 hrs. Stable in serum at 2-8°C up to 72 hrs

9

Creatinine

2 ml clotted blood.

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature or at 2-8°C up to 12hrs.Stable in serum at2-8°C up to 24hrs.

10

Sodium / Potassium /

Choloride.

2 ml clotted blood. Haemolyzed blood can not be used.

Do not collect blood from an arm receiving an I.V.infusion.

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature up to1hrs. Do not refrigerate sample before removing serum or plasma. Stable in serum or plasma at2-8°C up to 24hrs

12

Glucose

1ml anticoagulated blood (fasting, post-prandial,or random specimen)

Do not collect blood from an arm receiving an I.V.infusion.

Vial with yellow sticker

Stable in fluoride / oxalated blood at room temperature up to 3hrs. Stable in plasma at 2-8°C up to 48 hrs.

18

Phosphorus

2 ml clotted blood. Haemolyzed blood can not be used.

Plain Vial

With white

Sticker.

Stable at 2-8°C

for

48 Hr.

22

Protein, total.

2ml clotted blood.

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature up to 4hrs.

Stable in serum at room temperature for 4 hrs at 2-8°C for up to 3 days.

23

Thyroxine (T3)

2 ml clotted blood

Plain Vial

With white

Sticker.

Stable in serum at

2-8°c up to 48 hrs or at -20°C up to 30 days

24

Thyroxine (T4)

2 ml clotted blood

Plain Vial

With white

Sticker.

Stable in serum at

2-8°c up to 48 hrs or at -20°C up to 30 days

25

TSH

2 ml clotted blood

Plain Vial

With white

Sticker.

Stable in serum at

2-8°c up to 5days or at -20°C up to 30 days

28

Triglycerides

2 ml clotted blood(fasting specimen)

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature or at 2-8°C up to 3hrs

Stable in serum at

2-8°C up to 72hrs.

29

(Uric acid)

2 ml clotted blood.

Plain Vial

With white

Sticker.

Protect from daylight. stable in whole blood at room temperature up to 12 hrs.

Stable in serum at 2-8°C for up to 72 hrs.

30

Urea

2 ml clotted blood.

Plain Vial

With white

Sticker.

Stable in whole blood at room temperature or at 2-8°C up to 12hrs.

Stable in serum at 2-8°C for up to 48hrs.

31

Bence Jones protein

24 hrs urine preservative: Boric acid

10-15 gm.

5 liter plastic container. Provided by

Test as soon as possible. Refrigerated at 2-8°C if the test is not done within 1 hr.

32

Bilirubin

5-10 ml urine.

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

33

Glucose

5-10 ml urine.

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

34

Blood

5-10 ml urine

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

35

Ketones

5-10 ml urine

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

36

pH

5-10 ml midstream urine.

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

37

Protein

(Qualitative)

5-10 ml urine

Blue capped plastic

container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

36

Protein

(Quantitative)

24 hour urine.

Ref: send 5 ml well-mixed aliquot, kept cool, to reach destination within 24 hrs.

Boric acid 10 -15 gm.

5 liter plastic container. Provided by

Stable up to 24 hrs at 2-8°C.

38

Urobilinogen

5-10 ml urine.

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs.

Protect from light.

39

Occult blood

Collect a small sample

Patient should not eat red meat or vegetables containing citrus food for 3 days before the test.

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs

R/E

Small sample

Blue capped plastic container.

Provided by

Test as soon as possible.

Store refrigerated if testing is not done within 1 hrs

Out patient sample collection:

All collected samples are submitted to the respective divisions for testing

Special Timing of Collection:

SL.

TEST

PATIENT CONDITION

1

Any time any test of biochemistry except sugar and lipid profile

 Any time (P.F)

2

Glucose

Fasting for FBS

3

PP

PP two hours after meal or heavy breakfast

4

Random

Anytime

5

Lipid profile

12 hours fasting (minimum 9 hrs )

6

Coagulation profile

Any time (Mention history of drug in take )

7

HB, TC, DC, ESR, Platelet, Malaria Ag reticulocyte count, haemogram

Any time

8

Calcium

Any time (Preferably fasting)

9

Any serological test

Any time (preferably fasting)

10

, Microscopy and bio-chemistry

Any time

11

Urine Routine

Any time (preferably first morning sample)

12

Urine for culture

a) Any time b)Preferably first morning sample c)Mid-stream sample collected after cleaning the external genitalia

13

Stool for routine

Any time.

14

Stool for OBT

Any sample. The patient should avoid red meat, citrus foods, iron tonic & anti – inflammatory drug for 3 days

15

Urine for 24 hrs. Protein, Creatinine Clearance, Creatinine

Any time

Special handling needs between time of collection and time received by the Laboratory.

Transport of blood, urine and others fluids and tissue specimen from collection site to the laboratory is important and few precautions are necessary during this period.

    1. Specimen should be received by the laboratory staff within 30 minutes of collection to allow timely completion of specimen processing
    2. Avoid agitation of blood specimen to minimize hemolysis
    3. Specimen should be protected from direct exposure to light, which causes breakdown of certain analytes e.g. Bilirubin
    4. For analyses of unstable constituents, such as ammonia and acid phosphatase, specimens must be sent to the laboratory immediately after collection. Out side sample should be kept immediately at 4°C & transported on ice.
    5. All Laboratory specimens must be transported in a safe and convenient manner to prevent biohazard exposure or contamination of the specimen.
    6. Specimen requiring refrigeration must be maintained between 2ş C and 8ş C and can be carried in an insulated container
    7. Large volume urine specimen should be collected in a leak proof, 5L container.
    8. Stool should be transported in cardboard containers and placed in a polyethylene bag.

5. Identification of Patient and Collector Recording Procedure:

After sample collection all primary samples have to be properly labeled with Name, and ID. Urine fluids from different parts are collected in sterile disposable plastic containers or direct disposable syringe. Histopathology specimens are collected in plastic containers containing adequate amount of formalin. All containers are pre labeled. Respective technician or nursing staff writes the particular patient’s name, age, sex, ID number on the containers. The time and date of collection as well as their signature is present on a computerized requisition slip.

6. Safe Disposal of Laboratory Bio-medical Wastes :-

Sl no

Container/bagtype

Waste description

Responsibility & remarks

01

Black Bag

General Waste:

  • Office Papers
  • Paper Cups
  • Kitchen waste
  • Fruit peels

Please mention the method and the person / agency responsible for the disposal system

02

Yellow Bag

Infections Waste:

  • Human Tissue Organs & Body Parts
  • Animal Waste
  • Microbiological & Bio-technology Waste
  • Discarded cytotoxic medicines
  • Items contaminated with blood & body fluids of patients known to be HIV/HBV/HCV positive.
  • Dressings

03

BLUE - PUNCTURE PROOF CAN Containing 1% Sod hypo chlorite soln.

Sharp

  • Needles
  • Syringes
  • Scalpels
  • Blades etc.
  • Ampoules
  • Broken Glass
  • Slides
 

04.

RED Bag

Infectious Plastic Waste:

  • Disposable items like infected I.V tubings,

rubber catheters/ other catheters

  • Cannula
  • Cut Syringes
  • Cut Gloves
  • Drains
  • IV fluids bottles.
 

 

 

Do’S and Don’ts for waste Management

Do’s:-

Don’ts for Handling and Disposal of Hospital waste.

Safe handling of Chemicals

Dos and Don’ts for chemical treatment

Do’s:- ; Do apply to sharp or infected plastic waste----------

Do use 1% hypochlorite solution. Proper concentration is essential.

Do ensure all surfaces come in contract with chemical (including lumen).

Do let the contact time be at least 30 minutes.

Do change chemical solutions frequently with every shift.

Do handle with gloves and mask. Wear apron & boots if splashing is expected.

Don’ts:- Do not treat incinerable wastes chemically.

Recommendations for the prevention of Hepatitis B and HIV transmission among health care workers and waste handlers.

Health care workers are Doctors, Nurses, Students and Trainees whose activities involve contact with patients or with blood/ body fluids.

Specimens for whom Bio-Safety practices are required:

Bio-Safety practices:

A. Universal work precaution.

i) Hand washing

ii) Careful handling of sharp instruments

iii) Safe techniques

iv) Use of personal barrier precautions like gloves, masks, gowns, protective eye wears, foot wears.

B. Effective Disinfection & Sterilization

C. Management of SPILLS

 

D. Safe disposal of hospital wastes as discussed before.

E. Immunization against HB virus –All healthcare workers including waste handlers should be immunized with full course of Hepatitis B vaccine.

Storage of examined sample/Additional and repeat examination: After examination all samples are stored in respective department.

Storage period of examined specimen

The examined specimens are stored for re-examination and/ or additional tests for a minimum period as specified below

Clinical Biochemistry: 1 day at 2-80C

Hematology:

Complete Blood Counts: 24 hours at 2-80C

Coagulation screening test – 6-8 hours at 2-80C

Bone Marrow slides – 5 years

Clinical Pathology: Semen morphology slides -1 week

Histopathology:

Specimens – 15 days

Blocks – 5 years

Bone marrow aspirate and corresponding blood film and biopsy – 5 years

Cytopathology:

Fluids – 24 hours at 2-80C

Slides – 5 years

The additional test requested can be examined if sample is adequate and viability is

maintained.

 

 

 

 

 

 

 

 

 

Prevention and Management of needle stick injuries and accidents involving exposure to blood and body fluids

The cause of needle stick injury: -

The various procedure that have been reported to cause needle stick are

Site of injury :-

Hands are the most frequent (95 %) of injury particularly the dominant hand and the most frequently injured area is the palmer surface of the distal fore finger.

Population of risk: -

The improper use and disposal practices are the major reasons of the needle stick injuries – all over the world; nurses and physicians experience the highest incident of needle stick injuries.

Guidelines for Prevention of Needle Stick Injuries: -

HCW

Management

Definition-Exposure of the skin or mucous membrane to the blood or other body fluids from any patients.

First aid for all exposure.

  1. Skin puncture wounds from used and potentially contaminated needles or instrument should be encouraged to bleed and then washed thoroughly (but not scrubbed) with soap and water.
  2. Splashes of blood or body fluid into the mouth should wash out thoroughly with copious amounts of tap water.
  3. Splashes of blood or body fluids into the eyes should be well irrigated with a normal saline eyewash or running water.

Reporting Accidents

Action Plan.

  1. Subsequent action will be depend on the nature of the exposure and the likelihood of the "source" being a high risk group for hepatitis or HIV infection.
  2. When the source (usually a patient) of the contaminated sharp of material is known, blood should be taken for hepatitis screening and HIV antibody screening from the source.
  3. If the source belongs to a recognized high-risk group the administration of hepatitis – B immunoglobulin (HBIG) plus hepatitis B vaccine may be indicated before blood test results are known.

Hepatitis – B

  1. Source HBsAg -ve = No further action necessary.
  2. Source HBsAg +ve / unknown
    1. Exposed person already received a full course of hepatitis B vaccine

Level > 100 IU / ml à no further treatment required.

Level > 10, but < 100 IU / ml, give vaccine

Level < 10IU / ml, give vaccine booster

● Exposed person vaccinated but not complete full course

(No further action necessary but vaccine course should be completed at recommended intervals.

Level < 100 IU / ml

Give further dose and complete vaccine course.

HBIG will also be required if exposure is considerable.

Hepatitis C

There is prophylaxis or vaccination available against Hepatitis C. There is therefore no immediate action that needs to be taken following exposure to a possible hepatitis C source.

Exposed health care workers should be managed as follows:

  1. Known Hepatitis C

  1. Source known not to be infected with hepatitis C following testing at time of incident

Hepatitis C status of source unknown

Low risk – Obtain serum for anti HCV testing at 24 weeks

HCW found to be acquired hepatitis C infection following occupational exposure should be referred immediately to a specialist in infectious disease / physician for treatment.

Human Immunodeficiency Virus – HIV

This procedure applies to all possible exposures, whether they involve staff, patients.

If they’re us a risk of HIV exposure, individuals must be dealt with urgently to obtain prophylaxis as soon as possible and preferably within one hour

If the source is in a high-risk group or exposure is considerable, the source patient should be tested for HIV antibody. This requires informed consent to be given. These test are not performed out hours for this indication as the decision whether or not to start prophylaxis treatment must not be based solely of the results of an HIV antibody test and because any prophylaxis should be given within an hour of exposure.

For any exposure incident when the source patient is known it is recommended that Hepatitis B, C and HIV tests are carried out as a routine from the patient. For high-risk source or exposure these tests should be arranged as quickly as possible.

A blood sample must be taken from the injured or exposed person and sent to the microbiology laboratory to be stored. This will be used for future testing if necessary.

The risk of seroconversion following a single percutaneous exposure to HIV is only 0.3 % and following mucous membrane and skin exposure, 0.1 % and < 0.1 % respectively. A recent study suggested that this risk can be reduced still further if anti-retroviral drugs are taken prophylactically, starting as soon as possible after exposure, preferably within 1 hour. Since a combination of anti retroviral drugs will be more effective in suppressing in viral replication and reduced the risk of the development of resistance, a four-week course of triple anti retroviral post – exposure prophylaxis (PEP) is now recommended in certain circumstances.

Three types of exposure pose a risk: -

    1. The risk of transmission is increased with

    1. PEP should be considered whenever there has been exposure to material known to be, or strongly suspected to be, infected with HIV.
    2. High risk body fluids are: blood, amniotic fluid, vaginal secretions, semen, breast milk, CSF, peritoneal fluid, pericardial fluid, synovial fluid, unfixed tissues and organs and saliva in association with dental surgery. PEP should not be offered following contact through any route with urine, vomit, saliva and fasces unless they are visibly blood stained.
    3. If there is a risk of exposure to HIV, PEP must be started immediately and preferably within one hour. The following should be recommended:

It should be noted that these anti retroviral drugs are not licensed for incidents involving their use of prophylaxis against HIV infection. These drugs are prescribed on "a named patient basis.

 

Patient (exposed person) counseling and follow up for exposures assessed as significant and with a source known or strongly suspected to be HIV positive.

Initial post exposure management and counseling include:

    1. Post exposure follow up:
    1. The immediate PEP will be dispensed through the medical store.

 

 

 

 

 

 

 

 

 

Quick summarized note on NEEDLE PRICK INJURIES

 

Precaution:

1. Always wear gloves before the start of the procedure.

2. Be careful and concentrate on what you are doing.

 

Management:

1. Always note down the Pt’s. Name or Container I.D. No. and Take H/O Hepatitis or Infectious disease.

2. Allow bleeding for a short Period.

3. Wash with soap and water.

4. Apply pressure with pieces of cotton wool.

5. Disinfect the area with skin antiseptic.

6. Cover with BAND-AID / pieces of cotton wool soaked with Skin antiseptic.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

From https://www.amc.edu/pathology_labservices/addenda/addenda_documents/Americandiabetesassociationrecommendations2.pdf

Retrieved on 4/6/14

******************